1 Metabolic and regulatory rearrangements underlying glycerol metabolism in 2 Pseudomonas putida KT 2440 3 4 by 5 6

Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol.

BACKGROUND Given its high surplus and low cost, glycerol has emerged as interesting carbon substrate for the synthesis of value-added chemicals. The soil bacterium Pseudomonas putida KT2440 can use glycerol to synthesize medium-chain-length poly(3-hydroxyalkanoates) (mcl-PHA), a class of biopolymers of industrial interest. Here, glycerol metabolism in P. putida KT2440 was studied on the level o...

Knock - out of extracytoplasmic function sigma factor ECF - 10 affects stress resistance 1 and biofilm formation in Pseudomonas putida KT 2440

1 1 2 A second chromosomal copy of the catA gene endows Pseudomonas putida mt - 2 3 with an enzymatic safety valve for excess of catechol 4 5 by 6 7

1 2 Pseudomonas putida mt-2 harbors two different routes for catabolism of catechol, namely one meta 3 pathway encoded by the xyl genes of the TOL plasmid pWW0 and one ortho pathway determined by the 4 chromosomal ben and cat genes. P. putida mt-2 has a second chromosomal copy of the catA gene 5 (named catA2) located downstream of the ben operon that encodes an additional catechol-1,26 dioxygen...

The Glycerol-Dependent Metabolic Persistence of Pseudomonas putida KT2440 Reflects the Regulatory Logic of the GlpR Repressor

UNLABELLED The growth of the soil bacterium Pseudomonas putida KT2440 on glycerol as the sole carbon source is characterized by a prolonged lag phase, not observed with other carbon substrates. We examined the bacterial growth in glycerol cultures while monitoring the metabolic activity of individual cells. Fluorescence microscopy and flow cytometry, as well as the analysis of the temporal star...

The stereospecificity of sequential nicotinamide-adenine dinucleotide-dependent oxidoreductases in relation to the evolution of metabolic sequences.

The generalization that 'when a metabolic sequence involves consecutive nicotinamide-adenine dinucleotide-dependent reactions, the dehydrogenases have the same stereospecificity' was tested and confirmed for three metabolic sequences. (1) NAD+-xylitol (D-xylulose) dehydrogenase and NADP+-xylitol (L-xylulose) dehydrogenase are both B-specific. (2) D-Mannitol 1-phosphate dehydrogenase and D-sorbi...